EasySep Hu PSC Cardiomyocyte Enr Kit

From STEMCELL Technologies, this kit enables the isolation of untouched, highly purified human pluripotent stem cell (PSC)-derived cardiomyocytes from cell cultures by immunomagnetic negative selection. Non-cardiomyocytes are labeled with antibodies and magnetic particles, then separated without columns using an EasySep magnet so that the desired cells can simply be poured off into a new tube. The kit is designed to process up to 2.5 x 10^8 cells and can yield up to 99% purity.

The fast, easy-to-use protocol leaves isolated cells immediately available for downstream applications such as flow cytometry, culture, or DNA/RNA extraction. Recommended sample preparation involves dissociating human PSC-derived cardiomyocytes using the STEMdiff Cardiomyocyte Dissociation Kit, filtering aggregated suspensions through a pre-wetted 70 μm nylon mesh strainer, and resuspending cells at 2.5 x 10^7 cells/mL. STEMdiff Cardiomyocyte Support Medium is the recommended medium for the enrichment protocol. Components include an Enrichment Cocktail of monoclonal antibodies in PBS with 2% HPCD and 0.1% rHA, and EasySep Dextran RapidSpheres (a suspension of magnetic particles in water). Both should be stored at 2 - 8°C, not frozen, and remain stable until the expiry date on the label. The protocol is compatible with two magnets: the EasySep magnet (for 0.1 - 2 mL samples) and "The Big Easy" (for 0.1 - 8 mL samples).

Purity of the cTnT+ enriched fraction is typically 92.5 ± 7.6% (mean ± SD using the purple EasySep Magnet) when assessed by flow cytometry with an anti-human cardiac troponin T antibody. In a representative run, the start and final enriched fractions were 80.2% and 96.0% pure, respectively. For research use only.