EasySep Hu Progenitor w Platelet Deplete

This immunomagnetic cell isolation kit from STEMCELL Technologies uses the EasySep™ column-free approach to enrich human hematopoietic progenitor cells. It is designed for use with fresh or previously frozen cord blood and other cell preparations that contain large numbers of platelets. Unwanted cells are specifically labeled with dextran-coated magnetic particles using bispecific Tetrameric Antibody Complexes (TACs) that recognize both dextran and the unwanted cell surface antigens, then separated from unlabeled target cells using an EasySep™ magnet.

The kit comprises an enrichment cocktail and magnetic particles. The cocktail contains mouse IgG1 monoclonal antibodies directed against cell surface antigens on human blood cells, including CD2, CD3, CD11b, CD11c, CD14, CD16, CD19, CD24, CD56, CD61, CD66b, and glycophorin A. Inclusion of anti-CD61 enables depletion of platelets, megakaryocytes, and megakaryocytic progenitor cells. The procedure is compatible with the RoboSep™ automated system, the Purple EasySep™ Magnet, and "The Big Easy" Silver EasySep™ Magnet. Starting with nucleated cells, the typical CD34+ cell content of the enriched fraction typically ranges from 50 - 75%, depending on the quality of the starting sample. The reagents are stable at 2 - 8°C and are supplied for research use only under ISO 13485 quality standards.