EasySep Hu pDC Iso Kit-Sam

Plasmacytoid dendritic cells (pDCs) drive antiviral responses and immune tolerance, and isolating them from human blood requires a robust negative-selection workflow. The kit from STEMCELL Technologies depletes non-pDC leukocytes with immunomagnetic nanoparticles, leaving target cells untouched and free of antibody or magnetic beads for immediate downstream use.

Researchers in immunology and cell biology use this single-use kit for flow cytometry, cell culture, and functional assays, routinely achieving target purity above 90%. The configuration includes sample processing tubes and buffer for whole blood or leukapheresis input, supporting rapid enrichment without columns or specialized magnets beyond the standard EasySep setup.

Isolated p cells retain BDCA-2 and CD123 surface markers and remain viable for TLR stimulation studies, antigen presentation work, and co-culture experiments. Each vial provides reagents sufficient for processing a 50 uL to several ml starting volume, with refrigerated storage of unopened components to preserve activity until first use.