EasySep Hu Neutrophil Iso Kit

From STEMCELL Technologies, this kit enables researchers to isolate untouched, highly purified human neutrophils from fresh human peripheral blood leukocytes by immunomagnetic negative selection in as little as 14 minutes. The procedure is fast, easy-to-use, and column-free, using an EasySep™ magnet. Unwanted cells are labeled with antibodies and magnetic particles, and desired cells are simply poured off into a new tube, leaving isolated cells immediately available for downstream applications such as flow cytometry, culture, or DNA/RNA extraction. The cells remain viable and untouched by the labeling process.

Starting from whole blood prepared using HetaSep™ or Lymphoprep™ with RBC lysis, the neutrophil content (CD45+CD16+CD66b+) of the isolated fraction typically ranges from 98.7 ± 0.9% (mean ± SD), with up to 99% purity and the start and final isolated fractions reported at 52.0% and 99.0% respectively in the example shown. The kit targets non-neutrophils for removal using antibodies recognizing specific cell surface markers, and component reagents include a combination of monoclonal antibodies in PBS plus a suspension of magnetic particles in water; both are stored at 2 - 8°C and should not be frozen.

The kit is also supported on the company's automated cell separation platforms and is intended for research use only.