EasySep Hu EGFR Pos Sel Kit

This immunomagnetic positive selection kit from STEMCELL Technologies enables isolation of highly purified EGFR+ cells from fresh peripheral blood mononuclear cells, washed leukapheresis, or cell culture samples. The procedure labels desired cells with antibodies and magnetic particles, then separates them using an EasySep™ magnet without the need for columns. Unwanted cells are poured off while desired cells remain in the tube, and isolated cells are immediately available for downstream applications such as flow cytometry, culture, or DNA/RNA extraction.

The kit is designed for processing 1 x 10^9 cells and is reported to deliver up to 98% purity. In a typical isolation starting with 10% A549 cells spiked into human PBMCs, the EGFR+ cell content of the isolated fraction is 95.0 ± 2.8% (mean ± SD using the purple EasySep™ Magnet). The kit comprises two components: a Selection Cocktail containing monoclonal antibodies in PBS with 2% HPCD and 0.09% rHA, including an Fc receptor-blocking antibody, and EasySep™ Dextran RapidSpheres™ magnetic particles. Both components are stored at 2 - 8°C and may be shipped at room temperature (15 - 25°C).

Purity can be assessed by flow cytometry using Anti-Human CD45 Antibody, Clone HI30 and an anti-human EGFR antibody, clone EGFR.1. The product is intended for research use only and is not for human or animal diagnostic or therapeutic uses.