EasySep Hu Cen/Eff Mem CD4T Cell Iso Kit

From STEMCELL Technologies, this kit uses EasySep™ particle release technology to isolate particle-free human central (CD3+CD4+CD45RO+CD62L+) and effector (CD3+CD4+CD45RO+CD62L-) memory CD4+ T cells from human PBMCs or washed leukapheresis samples in as little as 38 minutes. The procedure is column-free and relies on immunomagnetic separation.

According to the manufacturer, starting with fresh PBMCs, the central memory CD4+ T cell content (CD3+CD4+CD45RO+CD62L+) of the isolated fraction is typically 92.3 ± 3.9% (mean ± SD using the purple EasySep™ magnet), and the effector memory content (CD3+CD4+CD45RO+CD62L-) of the isolated fraction is typically 92.4 ± 4.1% (mean ± SD). The manufacturer notes that RBCs were removed from the start sample by lysis prior to flow cytometry.