EasySep Hu CD45 Depletion Kit II - Sam

This negative-selection immunomagnetic kit from STEMCELL Technologies depletes human CD45+ leukocytes from single-cell suspensions, leaving non-hematopoietic populations untouched for downstream assay workflows. Built on the EasySep platform, it employs dextran-coated magnetic nanoparticles that bind antibody-labeled target cells and remove them within an external magnet, completing the depletion in roughly 8 minutes without columns. The format supports fresh or previously frozen human whole blood, peripheral blood mononuclear cell preparations, bone marrow, and dissociated tissue digests, typically processed in 1 mL aliquots with cell inputs up to 5 x 10^8 per separation. Researchers commonly apply the kit to enrich circulating tumor cells, isolate stromal and endothelial fractions, or prepare CD45-negative populations for flow cytometry and RNA sequencing analyses. Reagents are supplied in a single-use vial configuration and should be refrigerated upon arrival to preserve antibody activity across repeated aliquots.