EasySep Hu CD4+CD127loCD25+ Iso Kit-Sam

Immunomagnetic negative selection enriches viable human regulatory T cells from peripheral blood mononuclear cells, whole blood, or leukapheresis products using the EasySep platform. The kit targets the CD4+CD127loCD25+ Treg phenotype by labeling non-Treg leukocytes with a tetrameric antibody complex bound to dextran-coated magnetic nanoparticles, leaving untouched Tregs free for downstream suppression assays, in vitro expansion, flow cytometry characterization, and continued cell culture workflows.

STEMCELL Technologies supplies the antibody cocktail and magnetic particles in a single-use format that processes samples in standard round-bottom tubes held inside the EasySep magnet. Labeled cells migrate to the tube wall under magnetic force, while purified Tregs are simply poured off into a clean collection vessel. The column-free protocol is fast, gentle, and compatible with multiple starting sample preparations, preserving surface marker integrity and functional responsiveness without activating the cells of interest.

Investigators studying autoimmune disease, transplant tolerance, graft-versus-host disease, and cancer immunotherapy routinely use this research-use-only kit to obtain high-purity Tregs for functional suppression assays, phenotypic profiling, and gene expression studies. Reagents are supplied ready to use and should be stored refrigerated between uses to preserve reagent activity across the product shelf life.