EasySep Hu C/E Mem CD8T Cell Iso Kit-Sam

Magnetic negative-selection cell isolation targeting antigen-experienced CD8+ T lymphocyte subsets is delivered through this kit from STEMCELL Technologies. It enriches untouched central memory (CD45RO+ CCR7+) and effector memory (CD45RO+ CCR7-) populations from human peripheral blood mononuclear cells, whole blood, leukapheresis products, or buffy coat preparations, while depleting naive, TEMRA, and non-CD8 lineages. Output populations are immediately suitable for downstream flow cytometry, antigen-specific stimulation, intracellular cytokine staining, TCR sequencing, and adoptive transfer workflows in tumor immunology and vaccine research laboratories.

Running on the column-free EasySep RapidSpheres platform, the protocol typically completes in roughly 20 minutes at room temperature with a 1 mL sample volume, and a single separation handles up to 1 × 10⁸ leukocytes. The kit is supplied in a single-use vial format containing the enrichment antibody cocktail, magnetic RapidSpheres, and separation buffer, all refrigerated for in vitro research use only and stable through the printed expiration date.

Flexible input options support fresh, refrigerated, and previously cryopreserved PBMC sources, allowing integration into small pilot studies and larger translational cohorts exploring memory T cell persistence, exhaustion phenotypes, and checkpoint-blockade responses.