EasySep HLAChim WB B Pos Slctn Kit-Sam

Positive selection of B lymphocytes from human whole blood is the core capability of this EasySep kit from STEMCELL Technologies, supporting HLA chimerism monitoring after allogeneic hematopoietic stem cell transplantation. Antibody–magnetic nanoparticle complexes bind CD19+ B cells directly in the blood sample, eliminating the need for density gradient centrifugation or red cell lysis prior to separation.

The column-free workflow typically processes a small whole blood sample in roughly 20–30 minutes at room temperature using a compatible EasySep magnet, producing a viable B cell fraction suitable for downstream short tandem repeat PCR or next-generation sequencing chimerism testing. Researchers in transplant immunology laboratories rely on this human-specific positive selection approach to obtain purified B cells that retain surface marker integrity for follow-up flow cytometry purity checks.

Reagents are supplied as a single-use kit with components stored refrigerated and protected from light; the printed shelf life applies when the kit is handled as directed. Each unit is configured for transplant monitoring workflows where B cell chimerism provides a sensitive indicator of engraftment status.