EasySep HLA WB B Cell Enrichment Kit

STEMCELL Technologies' EasySep HLA WB B Cell Enrichment Kit uses immunomagnetic negative selection to isolate untouched human B cells directly from whole blood. The kit is intended for use with HetaSep-treated whole blood or washed leukapheresis samples, providing a preparatory step for downstream applications such as flow cytometry crossmatch and chimerism analysis.

Typical performance data show that, starting with HetaSep-treated whole blood, the CD19+ cell content of the enriched fraction typically ranges from 81.5 - 99.7% (gated on CD45+ cells). Starting with 0.5 mL of HetaSep-treated whole blood (equivalent to 5 mL of whole blood), 1.2 - 9.1 x 10e5 CD19+ cells are typically recovered. The kit is also compatible with PBMC, bone marrow, spleen, and lymph node samples, and the whole blood kit works on other red blood cell-containing samples including buffy coat, cord blood, and bone marrow.

For purity assessment of isolated cells, Anti-Human CD19, Clone HIB19 is the recommended staining antibody. STEMCELL Technologies' quality management system is certified to ISO 13485 medical device standards. The product is for research use only and is not intended for human or animal diagnostic or therapeutic uses.