EasySep Direct Hu T Cell Iso Kit

This immunomagnetic isolation kit from STEMCELL Technologies enables researchers to obtain highly purified human T cells directly from human whole blood, without the need for density gradient centrifugation, sedimentation, or red blood cell lysis. The workflow uses a negative selection strategy in which unwanted cells, including B cells, monocytes, granulocytes, and dendritic cells, are labelled with antibody complexes and magnetic particles and then separated using a column-free EasySep™ Magnet. The untouched T cells remain in suspension and can be collected by pouring or pipetting into a new tube, ready for downstream applications such as functional analysis, flow cytometry, culture, and nucleic acid extraction. Third-party product literature indicates the protocol can be completed in as little as 20 minutes.

The reagent cocktail includes a mouse (BALB/c) IgG1 monoclonal antibody directed against human and chimpanzee CD3, a mouse IgG2b monoclonal against human, rhesus, and cynomolgus CD4, and a mouse IgG1 monoclonal against human, rhesus, and cynomolgus CD8a.

According to the manufacturer's typical isolation profile, starting with human whole blood from normal healthy donors, the CD3+ content of the non-lysed final isolated fraction is 95.3 ± 1.4% (gated on CD45) or 94.9 ± 1.5% (not gated on CD45). In the worked example provided, the T cell (CD3+) content of the lysed whole blood start sample was 33.0% and rose to 95.0% in the non-lysed final isolated fraction (gated on CD45), with corresponding values of 33.0% and 94.9% when not gated on CD45. The starting T cell frequency in the non-lysed whole blood start sample in that example was 0.059%. The product is intended for research use only and is not approved for human or animal medical, clinical diagnostic, or food applications.