Anti-SARS-CoV S1 ProteinRBD,D005,UC100µL

This recombinant monoclonal antibody from STEMCELL Technologies, Clone D005, reacts with an epitope on the receptor-binding domain (RBD) of the S1 Spike Protein of SARS-CoV and SARS-CoV-2. It is a chimeric antibody composed of mouse variable regions and human IgG1 constant regions, expressed in HEK293 cells.

Unconjugated and of the IgG1 (human), kappa (mouse) isotype, the antibody was raised against recombinant SARS-CoV (isolate: WH20) Spike protein RBD comprising amino acids Arg306 - Phe527 fused to a C-terminal poly-His tag. It is purified by affinity chromatography and supplied in a phosphate-buffered solution, stable at -20°C when stored undiluted until the expiry date on the label.

The antibody reacts by ELISA with SARS-CoV Spike S1 Protein, SARS-CoV-2 (2019-nCoV) Spike RBD Protein, and SARS-CoV-2 (2019-nCoV) Spike S1 Protein, and does not cross-react with HCoV-229E, HCoV-HKU1 (isolate N1), HCoV-HKU1 (isolate N5), HCoV-NL63, HCoV-OC43 (Spike S1+S2 ECD), or MERS-CoV Spike S1 Proteins. It has been verified for interferometry and is reported for ELISA, flow cytometry, immunocytochemistry, immunofluorescence microscopy, immunohistochemistry, and immunoprecipitation. A suggested use range for ELISA is 1:5000 - 1:10,000, with titration recommended for optimal performance in each application.