Anti-Rt CD90, OX-7, FI, 100µg

STEMCELL Technologies offers a mouse monoclonal IgG1, kappa antibody (clone OX-7) conjugated to FITC, raised against rat thymocyte CD90 (Thy-1) antigen. The antibody reacts with rat CD90 (Thy-1) and mouse CD90.1 (Thy-1.1), the latter being an allelic form of CD90 expressed by mouse strains AKR/J, PL, and FVB/N, and also recognises guinea pig and rabbit. It does not react with CD90.2, which is expressed by many mouse strains including CBA and BALB/c.

CD90 is a GPI-linked membrane glycoprotein and member of the immunoglobulin superfamily. The 25 kDa core protein is N-glycosylated at three sites, giving rise to molecules with a range of molecular masses (25 - 37 kDa). In the rat, CD90 is expressed by hematopoietic stem cells, immature B cells, thymocytes, and neurons. In mouse strains expressing CD90.1, it is found on early-stage hematopoietic cells in the bone marrow, thymocytes, and circulating mature T cells. The OX-7 antibody has been reported to induce leukocyte activation, glomerular nephritis, apoptosis in glomerular mesangial cells, and vascular permeability.

Verified for flow cytometry, the clone has also been validated for purity assessments of cells isolated from compatible mouse strains using EasySep kits, including the EasySep Mouse T Cell Isolation Kit. The reagent is formulated in a phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.5% (w/v) bovine serum albumin, purified by affinity chromatography and conjugated under optimal conditions; the solution is free of unconjugated FITC. The suggested use is ≤ 0.25 μg per 1 x 10^6 cells in 100 μL volume. The product is stable at 2 - 8 °C when stored undiluted; do not freeze and protect from prolonged exposure to light.