Anti-Ms NK1.1, PK136, AZ, 25µg

An antibody from STEMCELL Technologies, clone PK136, reacts with murine NK1.1 (CD161), an approximately 80 kDa homodimeric type 2 transmembrane glycoprotein and C-type lectin receptor. NK1.1 is expressed on NK cells, NK-T cells, and rare subsets of T cells and monocytes in select mouse strains.

The NK1.1 antigen is encoded by the Klrb1b and Klrb1c genes, which specify the CD161b and CD161c polypeptides. CD161b is expressed only by Swiss NIH and SJL mice, while CD161c is expressed by strains such as C57BL, FVB/N, and NZB, but not by A, AKR, BALB/c, CBA/J, C3H, C57BR, C58, DBA/1, DBA/2, NOD, SJL, or 129. NK1.1 has functional roles in NK cell activation and proliferation, induction of interferon-γ production, and release of cytotoxic granules, with its cell-surface expression specifically upregulated by IL-12.

The antibody is of mouse IgG2a, kappa isotype and is offered in multiple conjugations including Alexa Fluor 488, APC, Biotin, FITC, PE, PerCP-Cy5.5, and unconjugated. Reported applications include flow cytometry, cell separation, CyTOF, depletion, functional assays, immunocytochemistry, immunofluorescence, immunohistochemistry, and immunoprecipitation. For detection of NK cells in NK1.1-negative strains, clone DX5 is recommended.