Anti-Ms NK1.1, PK136, AD, 25µg

STEMCELL Technologies supplies this Alexa Fluor® 488-conjugated mouse monoclonal antibody, clone PK136, of the IgG2a, kappa isotype, raised in BALB/c mice using NK-1+ cells from mouse spleen and bone marrow as the immunogen. The reagent reacts with murine NK1.1 (CD161), an approximately 80 kDa homodimeric type 2 transmembrane glycoprotein and C-type lectin receptor expressed on NK cells, NK-T cells, and rare subsets of T cells and monocytes in select strains of mice. NK1.1 is encoded by the Klrb1b and Klrb1c genes, which specify the CD161b and CD161c polypeptides. CD161b is expressed by Swiss NIH and SJL mice, while CD161c is expressed by strains such as C57BL, FVB/N, and NZB, but not by A, AKR, BALB/c, CBA/J, C3H, C57BR, C58, DBA/1, DBA/2, NOD, SJL or 129.

The antigen has functional roles in modulating NK cell activation and proliferation, inducing interferon-γ production, and triggering the release of cytotoxic granules, with its cell surface expression specifically upregulated by IL-12. Flow cytometry (FC) is listed as both a verified and reported application.

Provided at 0.5 mg/mL in a phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide, the antibody was purified by affinity chromatography and the solution is free of unconjugated Alexa Fluor® 488. For flow cytometry, the suggested use is ≤ 0.25 μg per 1 x 10e6 cells in 100 μL, with titration recommended for optimal performance. The product is stable at 2 - 8°C when stored undiluted; do not freeze and protect from prolonged exposure to light.