Anti-Ms MHC ClassII,M5/114.15.2,FI,500µg

STEMCELL Technologies provides this FITC-conjugated rat monoclonal IgG2b, kappa antibody (clone M5/114.15.2) directed against the mouse major histocompatibility complex class II receptor (MHC-II). MHC-II is a heterodimeric transmembrane glycoprotein comprising an α subunit non-covalently associated with a β subunit; in mice, MHC-II complexes are designated I-A and I-E and comprise Aα/Aβ and Eα/Eβ heterodimers, respectively. Together with the CD3/T cell receptor (TCR) complex and CD4 molecules, MHC-II mediates the presentation of peptides generated from hydrolysis of exogenous antigens by antigen-presenting cells to CD4+ T (helper) cells, either suppressing or inducing an immune response to those peptides.

The M5/114.15.2 antibody reacts with an extracellular epitope, recognizing a polymorphic determinant shared by the I-Ab, I-Ad, I-Aq, I-Ed and I-Ek (but not I-Af, I-Ak or I-As) MHC class II alloantigens expressed on B cells, dendritic cells, macrophages, monocytes and activated T cells of mice carrying the H-2b, d, p, q, r and u haplotypes. It does not react with cells from NOD (H-2g7) mice. Raised in a Rat (BN x LEW) host using a mixture of activated C57BL/6 mouse splenocytes and anti-irradiated BN rat lymphoma-derived lymphocytes as the immunogen, the reagent has been verified and reported for flow cytometry (FC) and used for purity assessments of cells isolated with the EasySep™ Mouse CD11c Positive Selection Kit II. It is supplied in phosphate-buffered saline (pH 7.2) containing 0.09% sodium azide and 0.1% gelatin, purified by affinity chromatography and conjugated with FITC under optimal conditions, free of unconjugated FITC. For flow cytometry, the suggested use is ≤ 0.25 μg per 1 × 10⁶ cells in 100 μL, with titration recommended for optimal performance.