Anti-Ms Ly-6G, 1A8, PS, 25µg

This PerCP-Cy5.5-conjugated antibody from STEMCELL Technologies is a rat monoclonal antibody (clone 1A8, isotype IgG2a, kappa) raised against mouse Ly-6G. Ly-6G is a 21 - 25 kDa GPI-anchored protein which, together with the structurally related Ly-6C protein, comprises the granulocyte receptor-1 antigen (Gr-1). Gr-1 expression is found on monocytes, neutrophils, and subsets of macrophages, plasmacytoid dendritic cells, and T cells; in the periphery Gr-1 is found predominantly on neutrophils, where it serves as a useful marker for these cells.

The 1A8 clone binds specifically to Ly-6G, distinguishing it from the commonly used RB6-8C5 antibody, which recognizes both Ly-6G and Ly-6C. As a result, the 1A8 antibody detects Ly-6G-expressing granulocytes in peripheral blood, whereas RB6-8C5 also binds Ly-6C-expressing lymphocytes, monocytes, and dendritic cells. The reagent has been verified for flow cytometry and has been used to assess the purity of cells isolated with EasySep™ Mouse Neutrophil Enrichment Kit. For flow cytometry applications, the suggested use is ≤ 0.25 μg per 1 x 10^6 cells in 100 μL, with titration recommended for each application to determine optimal performance.

The antibody was purified by affinity chromatography and conjugated with PerCP-Cy5.5 under optimal conditions, and the solution is free of unconjugated PerCP-Cy5.5. It is formulated in phosphate-buffered saline (pH 7.2) containing 0.09% sodium azide and 0.1% gelatin. The product should be stored undiluted at 2 - 8°C, protected from prolonged exposure to light, and not frozen. As with all STEMCELL Technologies antibody products, it is intended for research use only and is not intended for human or animal diagnostic or therapeutic uses unless otherwise stated.