Anti-Ms EPCR, RMEPCR1560, FI, 25tests

A rat monoclonal antibody from STEMCELL Technologies, Clone RMEPCR1560 (1560), is conjugated to FITC and directed against mouse EPCR (CD201). The target is an ~25 kDa type I transmembrane glycoprotein expressed by endothelial cells, subsets of hematopoietic stem cells (HSCs) and dendritic cells, and several malignant cell lines, and is also found in a soluble form in plasma. EPCR shows homology with the MHC class 1/CD1 protein family and binds protein C and activated protein C, augmenting protein C activation by the thrombin-thrombomodulin complex and regulating blood coagulation and inflammation.

EPCR protein expression has been detected on ~1.5% of mouse bone marrow cells, and purified EPCR+ cells are highly enriched for HSC activity as evidenced by high in vivo repopulation activity. The antibody is of the IgG2b, kappa isotype, was raised against the soluble form of mouse EPCR protein, and has been verified for flow cytometry. For flow cytometry, the suggested use is 20 μL per 1 x 10^6 cells in 100 μL or per 100 μL of whole blood; titration is recommended for optimal performance in each application. The product is not recommended for use with mouse bone marrow cells.

The reagent is supplied as a liquid in phosphate-buffered saline containing < 0.1% (w/v) sodium azide and < 0.1% (w/v) bovine serum albumin, purified by affinity chromatography and conjugated with FITC under optimal conditions. It is stable at 2 - 8°C when stored undiluted; do not freeze and protect from prolonged exposure to light. According to the manufacturer's safety data sheet, the chemical is not classified as hazardous according to the 2012 OSHA Hazard Communication Standard (29 CFR 1910.1200) or the Globally Harmonized System (GHS).