Anti-Ms EPCR, RMEPCR1560, FI, 100tests

STEMCELL Technologies offers a rat monoclonal IgG2b, kappa antibody conjugated with FITC that specifically recognizes the mouse endothelial protein C receptor (EPCR), also designated CD201. The antibody, identified as clone RMEPCR1560 (1560), was raised against the soluble form of mouse EPCR protein and purified by affinity chromatography prior to fluorophore conjugation.

EPCR is described as an approximately 25 kDa type I transmembrane glycoprotein expressed by endothelial cells, subsets of hematopoietic stem cells, dendritic cells, and several malignant cell lines; it is also present in a soluble form in plasma. The receptor exhibits homology with the MHC class 1/CD1 protein family and binds protein C and activated protein C, contributing to protein C activation by the thrombin-thrombomodulin complex and to the regulation of blood coagulation and inflammation. The manufacturer states that EPCR protein expression has been detected on approximately 1.5% of mouse bone marrow cells, and that purified EPCR-positive cells are highly enriched for hematopoietic stem cell activity.

The antibody is verified for flow cytometry (FC) applications and is described as suitable for cell isolation. It is supplied in phosphate-buffered saline containing less than 0.1% (w/v) sodium azide and less than 0.1% (w/v) bovine serum albumin. Storage guidance specifies that the product remains stable at 2 - 8°C when stored undiluted; it should not be frozen and should be protected from prolonged exposure to light. The suggested usage for flow cytometry is 20 µL per 1 × 10^6 cells in 100 µL, or per 100 µL of whole blood, with titration recommended for optimal performance. The manufacturer notes that this antibody is not recommended for use with mouse bone marrow cells.