Anti-Ms CD90.2, 53-2.1, UC, 500µg

A rat monoclonal IgG2a, kappa antibody from STEMCELL Technologies, clone 53-2.1, recognizes mouse CD90.2 (Thy-1.2), an ~25 - 35 kDa glycophosphatidylinositol (GPI)-anchored glycoprotein. The unconjugated reagent was raised against mouse thymus or spleen cells and is reported to block the binding of antibody clone 30-H12.

CD90.2 is expressed at high levels on the surface of thymocytes, most peripheral T cells, and lymphatic endothelial cells, and at lower levels on hematopoietic stem cells, some intraepithelial T lymphocytes, fibroblasts, and neurons. It is an allelic form of the CD90 antigen expressed by mouse strains including BALB/c, C3H/He, C57BL/6, C58/, CBA/J, DBA, NZB/L, and SJL. The clone does not react with CD90.1, which is expressed by mouse strains such as AKR/J, PL, and FVB/N, or with rat CD90. The antigen has been shown to be involved in costimulation of lymphocyte proliferation, as well as in hematopoietic stem cell differentiation and thymocyte adhesion.

The antibody is formulated in a phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide, and has been purified by affinity chromatography. Flow cytometry has been verified as an application, with reported uses including depletion, electron microscopy, functional assay (FA), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and radioimmunoassay (RIA). For flow cytometry, the suggested use is ≤ 5 µL per 1 x 10^6 cells in 100 µL volume or per 100 µL whole blood, and titration is recommended for optimal performance. The product is stable at 2 - 8°C when stored undiluted; do not freeze.