Anti-Ms CD90.2, 53-2.1, FI, 500µg

This FITC-conjugated reagent from STEMCELL Technologies is a rat monoclonal antibody (clone 53-2.1, IgG2a, kappa) raised against mouse thymus or spleen cells. It binds CD90.2 (Thy-1.2), an approximately 25–35 kDa glycophosphatidylinositol (GPI)-anchored glycoprotein also known by the alternative names T25, Thy 1b, and Thymus cell antigen 1 theta. The antibody reacts with mouse strains including BALB/c, C3H/He, C57BL/6, C58, CBA/J, DBA, NZB/-, and SJL, but does not recognize CD90.1 (Thy-1.1) or rat CD90.

The target antigen is expressed at high levels on thymocytes, most peripheral T cells, and lymphatic endothelial cells, and at lower levels on hematopoietic stem cells, some intraepithelial T lymphocytes, fibroblasts, and neurons. CD90.2 has been implicated in costimulation of lymphocyte proliferation, hematopoietic stem cell differentiation, and thymocyte adhesion, and the 53-2.1 clone reportedly blocks binding of clone 30-H12. Flow cytometry is the verified application; immunocytochemistry, immunofluorescence, and immunohistochemistry have been reported in the literature. The antibody has also been verified for purity assessments of cells isolated with EasySep™ Mouse T Cell, CD4+ T Cell, and CD8+ T Cell Isolation Kits.

The product is supplied as a phosphate-buffered solution at pH 7.2 containing 0.09% sodium azide, purified by column chromatography, and is stable at 2–8°C when stored undiluted; it should not be frozen and should be protected from prolonged exposure to light. For flow cytometry, the suggested use is ≤ 0.25 μg per 1 × 10^6 cells in 100 μL, with titration recommended for optimal performance in each application. It is intended for research use only.