Anti-Ms CD90.2, 53-2.1, BT, 500µg

A biotinylated monoclonal antibody from STEMCELL Technologies, clone 53-2.1, that specifically recognizes mouse CD90.2 (Thy-1.2), an ~25–35 kDa glycophosphatidylinositol (GPI)-anchored glycoprotein expressed at high levels on the surface of thymocytes, most peripheral T cells, and lymphatic endothelial cells, and at lower levels on hematopoietic stem cells, some intraepithelial T lymphocytes, fibroblasts, and neurons.

CD90.2 is an allelic form of the CD90 antigen expressed by mouse strains including BALB/c, C3H/He, C57BL/6, C58/, CBA/J, DBA, NZB/-, and SJL. The 53-2.1 antibody does not react with CD90.1, which is expressed by strains such as AKR/J, PL, and FVB/N, or with rat CD90. CD90.2 has been shown to be involved in costimulation of lymphocyte proliferation, as well as in hematopoietic stem cell differentiation and thymocyte adhesion. The 53-2.1 antibody reportedly blocks the binding of antibody clone 30-H12.

The reagent is supplied as a clear liquid and is not classified as a hazardous substance under the Globally Harmonized System. Suitable applications include flow cytometry, immunofluorescence, magnetic and fluorescent cell sorting, functional assays, immunohistochemistry, immunoprecipitation, electron microscopy, and depletion studies. The host species is rat and the isotype is IgG2a, kappa. The reagent should be stored at 2–8 °C, protected from light, and not frozen; freeze-thaw cycles should be minimized.