Anti-Ms CD90.2, 53-2.1, AZ, 100µg

STEMCELL Technologies offers the 53-2.1 monoclonal antibody, a rat-derived primary antibody of the IgG2a, kappa isotype, directed against mouse CD90.2 (Thy-1.2). The target antigen is an approximately 25–35 kDa glycophosphatidylinositol (GPI)-anchored glycoprotein (Gene ID: 21838) expressed at high levels on thymocytes, most peripheral T cells, and lymphatic endothelial cells, and at lower levels on hematopoietic stem cells, some intraepithelial T lymphocytes, fibroblasts, and neurons.

CD90.2 is an allelic form of the CD90 antigen found in mouse strains such as BALB/c, C3H/He, C57BL/6, C58/, CBA/J, DBA, NZB/-, and SJL. The 53-2.1 antibody does not cross-react with CD90.1 (expressed by strains including AKR/J, PL, and FVB/N) or with rat CD90. The antigen has been implicated in costimulation of lymphocyte proliferation, hematopoietic stem cell differentiation, and thymocyte adhesion, and the 53-2.1 antibody reportedly blocks the binding of clone 30-H12.

Documented applications include flow cytometry, immunohistochemistry, immunofluorescence, immunoprecipitation, electron microscopy, cell depletion, and functional assays, with relevance to immunology research. Available conjugation formats across the product line include APC, biotin, FITC, PE, and unconjugated.