Anti-Ms CD62L, MEL-14, PS, 25µg

This rat monoclonal IgG2a, kappa antibody, supplied by STEMCELL Technologies, targets murine CD62L (L-selectin), an ~74 - 95 kDa single-chain type I glycoprotein expressed on the surface of a majority of leukocytes. CD62L is a member of the selectin protein family and mediates leukocyte-endothelial cell interactions through its association with ligands including CD34, glyCAM-1, MAdCAM-1, and PSGL-1. It functions to facilitate lymphocyte rolling on the vascular endothelium, trafficking of lymphocytes to lymph nodes, and homing of lymphocytes and neutrophils to sites of inflammation.

The MEL-14 clone was raised against the 38C-13 B cell lymphoma derived from a C3H/eb mouse. Murine CD62L is expressed on most thymocytes, with the highest expression levels on subsets of immunocompetent or dividing progenitor cells, as well as on neutrophils, eosinophils, monocytes, and subsets of B, T, and NK cells. CD62L is rapidly cleaved and shed from lymphocytes and neutrophils upon cellular activation, so the antibody may be used together with other cell surface markers to distinguish naïve, memory, and effector T cells based on differences in CD62L expression. The antibody has been shown to block migration of lymphocytes to lymph nodes and to inhibit leukocyte rolling. The associated gene ID is 20343.

The antibody is conjugated to PerCP-Cy5.5 and flow cytometry (FC) is the verified application. For flow cytometry, the suggested use is ≤ 0.25 µg per 1 x 10^6 cells in 100 µL volume, with titration recommended for optimal performance. The antibody was purified by column chromatography and is supplied in an aqueous buffer containing 0.09% sodium azide, which may contain carrier protein/stabilizer. The product is stable at 2 - 8°C when stored undiluted; it should not be frozen and should be protected from prolonged exposure to light. This antibody clone has been verified for purity assessments of cells isolated with EasySep™ kits.