Anti-Ms CD62L, MEL-14, PE, 100µg

From STEMCELL Technologies, this PE-conjugated rat monoclonal antibody (clone MEL-14) reacts with murine CD62L (also known as L-Selectin or LECAM-1), an approximately 74–95 kDa type I glycoprotein expressed on the surface of a majority of leukocytes. The reagent is intended for flow cytometry and immunostaining to detect CD62L on murine lymphocytes, where CD62L is used as a marker of naïve and central memory T cells.

CD62L mediates leukocyte–endothelial cell interactions through its association with ligands including CD34, glyCAM-1, MAdCAM-1 and PSGL-1, and functions to facilitate lymphocyte rolling on the vascular endothelium, trafficking of lymphocytes to the lymph nodes, and homing of lymphocytes and neutrophils to sites of inflammation. Murine CD62L is expressed on most thymocytes, with the highest expression levels being found on subsets of immunocompetent or dividing progenitor cells, as well as on neutrophils, eosinophils, monocytes, and subsets of B, T and NK cells. The MEL-14 antibody may be used together with antibodies to other cell surface markers to distinguish naïve, memory and effector T cells, and has been shown to block migration of lymphocytes to lymph nodes and to inhibit leukocyte rolling.

The antibody is of the rat IgG2a, kappa isotype and is reactive against mouse CD62L. Reported uses include purity assessment of neutrophils and monocytes, with applications spanning flow cytometry, immunocytochemistry, immunofluorescence, immunohistochemistry, immunoprecipitation, CyTOF and functional assays. Recommended storage is 2–8°C, protected from light, with avoidance of repeated freeze–thaw cycles.