Anti-Ms CD62L, MEL-14, BT, 50µg

A rat monoclonal IgG2a, kappa antibody from STEMCELL Technologies, Clone MEL-14, targets murine CD62L (also known as L-Selectin or LECAM-1), an approximately 74–95 kDa type I glycoprotein. The product is offered in a biotin-conjugated format and reacts with CD62L expressed on the surface of a majority of leukocytes.

CD62L mediates leukocyte–endothelial cell interactions through its association with ligands such as CD34, glyCAM-1, MAdCAM-1, and PSGL-1. It facilitates lymphocyte rolling on the vascular endothelium, trafficking of lymphocytes to lymph nodes, and homing of lymphocytes and neutrophils to sites of inflammation. In the mouse, CD62L is expressed on most thymocytes—with the highest levels on subsets of immunocompetent or dividing progenitor cells—as well as on neutrophils, eosinophils, monocytes, and subsets of B, T, and NK cells. The MEL-14 antibody can be used alongside other surface markers to distinguish naïve, memory, and effector T cell populations, and has been shown to block lymphocyte migration to lymph nodes and inhibit leukocyte rolling. The antibody has also been verified for purity assessment of neutrophils and monocytes.

Validated applications include flow cytometry (demonstrated on C57BL/6 mouse splenocytes), immunocytochemistry, immunofluorescence, immunohistochemistry, immunoprecipitation, CyTOF, and functional assays. The product supports research workflows such as immunomagnetic negative isolation of untouched mouse naïve CD4+ T cells, pan-naïve T cells, and naïve CD8+ T cells.