Anti-Ms CD49f, GoH3, AZ, 25tests

From STEMCELL Technologies, this rat monoclonal IgG2a, kappa antibody (clone GoH3) is conjugated to APC (Allophycocyanin) and directed against CD49f (integrin α6), an ~150 kDa transmembrane glycoprotein. CD49f associates non-covalently with CD29 (integrin β1) or CD104 (integrin β4) to form the heterodimeric receptors VLA-6 and α6β4, which bind the extracellular matrix protein laminin. The target is a disulfide-linked dimer comprising an ~120 kDa heavy chain and an ~30 kDa membrane-bound light chain, with splice variants that affect the cytoplasmic domain. CD49f is expressed on T cells, monocytes, platelets, placental trophoblasts, epithelial cells, and endothelial cells.

The antibody was generated using Mouse (BALB/c) mammary tumor cells as the immunogen and shows reactivity with Human, Mouse, Rhesus, Cynomolgus, Baboon, Chimpanzee, Capuchin Monkey, Cat, Cow, Dog, Horse, Pig, Rabbit, and Sheep. The GoH3 clone reacts with an extracellular epitope on CD49f and reportedly blocks integrin α6 function in vivo and binding of integrin α6 to laminin in vitro. The reagent is verified for flow cytometry, with a suggested use of ≤ 5 μL per 1 x 10^6 cells in 100 μL.

The product is supplied in a phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) bovine serum albumin, and was purified by affinity chromatography prior to conjugation with APC. It should be stored undiluted at 2 - 8°C; do not freeze and protect from prolonged exposure to light.