Anti-Ms CD49f, GoH3, AD, 25tests

This Alexa Fluor® 488-conjugated monoclonal antibody from STEMCELL Technologies is a rat IgG2a, kappa antibody that recognizes CD49f (integrin α6), an approximately 150 kDa transmembrane glycoprotein. CD49f associates non-covalently with CD29 (integrin β1) or CD104 (integrin β4) to form the heterodimeric receptors VLA-6 and α6β4, which bind the extracellular matrix protein laminin. The antibody, produced using the GoH3 clone from mouse (BALB/c) mammary tumor cells, reacts with an extracellular epitope on CD49f and has been reported to block integrin α6 function in vivo and laminin binding in vitro.

CD49f is a disulfide-linked dimer comprising an approximately 120 kDa heavy chain and an approximately 30 kDa membrane-bound light chain, and is expressed on T cells, monocytes, platelets, placental trophoblasts, epithelial cells, and endothelial cells. It is involved in cell adhesion and in regulating signaling pathways related to T-cell activation and proliferation, as well as the differentiation and maintenance of stem cell pluripotency. CD49f is considered the most important marker for selecting mouse mammary stem and progenitor cells.

The reagent is supplied as a phosphate-buffered solution (pH 7.2) containing 0.09% sodium azide and 0.2% (w/v) bovine serum albumin, and has been purified by affinity chromatography and conjugated with Alexa Fluor® 488. It is verified for flow cytometry, with reported use in immunofluorescence and immunohistochemistry, and has been validated for labeling hematopoietic cells cultured in StemSpan™ media. For flow cytometry the suggested use is 5 µL per 1 × 10⁶ cells in 100 µL volume or per 100 µL of whole blood. The product should be stored at 2–8 °C, protected from prolonged light exposure, and not frozen.