Anti-Ms CD49f, GoH3, 100µg

A rat monoclonal IgG2a, kappa antibody against CD49f (integrin α6), manufactured by STEMCELL Technologies. Clone GoH3 was raised in a Rat (SD) host against mouse (BALB/c) mammary tumor cells and recognizes an extracellular epitope on the CD49f antigen. The antibody has demonstrated reactivity with human, mouse, rhesus, cynomolgus, baboon, chimpanzee, capuchin monkey, cat, cow, dog, horse, pig, rabbit, and sheep samples.

CD49f is an ~150 kDa transmembrane glycoprotein that forms a disulfide-linked dimer comprising an ~120 kDa heavy chain and an ~30 kDa membrane-bound light chain. It associates non-covalently with CD29 (integrin β1) or CD104 (integrin β4) to form the heterodimeric receptors VLA-6 and α6β4, which bind the extracellular matrix protein laminin. The GoH3 antibody reportedly blocks integrin α6 function in vivo and binding of integrin α6 to laminin in vitro. CD49f is expressed on the surface of T cells, monocytes, platelets, placental trophoblasts, epithelial and endothelial cells, and is considered the most important marker for selecting mouse mammary stem and progenitor cells.

The antibody is verified for flow cytometry (FC) and has been reported for immunofluorescence microscopy (IF) and immunohistochemistry (IHC). It has been used to label hematopoietic cells grown in StemSpan™ SFEM and StemSpan™ H3000 media. The suggested use for flow cytometry is 5 μL per 1 × 10⁶ cells in 100 μL volume or per 100 μL of whole blood; titration is recommended for optimal performance in each application.