Anti-Ms CD49b, DX5, PE, 50µg

STEMCELL Technologies offers a rat monoclonal antibody (Clone DX5) of the IgM, kappa isotype, conjugated to PE and raised against mouse CD49b (Integrin α2). CD49b is an ~150 kDa type I transmembrane glycoprotein that associates non-covalently with CD29 (integrin β1) to form the VLA-2 heterodimer, a receptor for extracellular matrix proteins including collagen, E-cadherin, fibronectin and laminin. The antibody is verified for flow cytometry and cell separation applications, and the clone is particularly useful for identifying NK cells in mouse strains that lack the NK1.1 antigen.

According to the product information, CD49b is highly expressed by platelets and is found on a majority of NK cells, on NKT cells, and on a small subset of CD8+ T cells, as well as on several tissues including intestine, kidney, mammary gland and lung. Binding of the DX5 antibody has not been observed to affect VLA-2 integrin function, although DX5 binding is blocked by the clone HMα2 antibody. For flow cytometry, the suggested use is ≤ 0.25 μg per 1 × 10^6 cells in a 100 μL volume, and titration is recommended for optimal performance. The antibody clone has been verified for purity assessments of cells isolated with the EasySep™ Mouse NK Cell Enrichment Kit.

The product is supplied as a phosphate-buffered solution at pH 7.2 containing 0.09% sodium azide, and the PE-conjugated antibody is reported to be at > 85% purity, free of unconjugated PE and unconjugated antibody. It should be stored undiluted at 2 - 8 °C, protected from prolonged exposure to light and not frozen. The safety data sheet classifies the mixture as not a hazardous substance or mixture according to the GHS; it is a clear liquid. The product is for research use only and is not intended for human or animal diagnostic or therapeutic applications.