Anti-Ms CD49b, DX5, FI, 500µg

This anti-mouse CD49b antibody, clone DX5, is a rat monoclonal IgM reagent conjugated to FITC and manufactured by STEMCELL Technologies for research use only. The antibody targets CD49b (integrin α2), an approximately 150 kDa type I transmembrane glycoprotein that pairs non-covalently with CD29 (integrin β1) to form the VLA-2 heterodimer, a receptor for extracellular matrix proteins including collagen, E-cadherin, fibronectin, and laminin.

The clone is widely used to identify NK cells in mice that lack the NK1.1 antigen. It recognizes CD49b expressed on platelets, a majority of NK cells, NKT cells, and a small subset of CD8+ T cells, as well as on tissues including intestine, kidney, mammary gland, and lung. Binding has not been observed to affect VLA-2 integrin function, though DX5 binding is blocked by the HMα2 clone. The antibody was raised against IL-2-propagated NK1.1+ cells from C57BL/6 mice, purified by affinity chromatography, and is supplied free of unconjugated FITC in a phosphate-buffered solution at pH 7.2 containing 0.09% sodium azide.

Flow cytometry is the verified application, with a suggested use of ≤ 1 µg per 1 × 10⁶ cells in 100 µL; titration is recommended for optimal performance for each application. The product is stable at 2 - 8 °C when stored undiluted; it should not be frozen and must be protected from prolonged exposure to light. The reagent appears as a clear liquid and is not classified as hazardous under GHS or the 2012 OSHA Hazard Communication Standard (29 CFR 1910.1200).