Anti-Ms CD49b, DX5, 500µg

STEMCELL Technologies offers this rat IgM, κ monoclonal antibody (clone DX5) specific for mouse CD49b (also known as Integrin α2, α2 integrin, or the VLA-2 α chain). The unconjugated reagent recognises an ~150 kDa type I transmembrane glycoprotein that associates non-covalently with CD29 (integrin β1) to form the VLA-2 heterodimer, a receptor for extracellular matrix proteins such as collagen, E-cadherin, fibronectin and laminin.

CD49b is expressed by a majority of NK cells, by NKT cells, by platelets, and by a small subset of CD8+ T cells that increases substantially following viral infection, as well as by several tissues including the intestine, kidney, mammary gland and lung. Clone DX5 is particularly useful for identifying NK cells in mice lacking the NK1.1 antigen, and is suitable for flow cytometry and cell separation; immunohistochemistry (IHC) has also been reported. Binding of the DX5 antibody has not been observed to affect VLA-2 integrin function.

The antibody was raised against IL-2-propagated NK1.1+ cells from C57BL/6 mice and is provided at >85% purity in a phosphate-buffered solution (pH 7.2) containing 0.09% sodium azide. For flow cytometry the manufacturer recommends ≤0.25 µg per 1 × 10^6 cells in 100 µL and titration for each application. DX5 binding is blocked by the HMα2 antibody. Store undiluted at 2-8°C; do not freeze. For research use only.