Anti-Ms CD45R, RA3-6B2, PS, 100µg

Rat monoclonal IgG2a antibody (clone RA3-6B2) targeting the exon A-restricted isoform of mouse CD45, also known as CD45R or B220. Manufactured by STEMCELL Technologies, this reagent is conjugated to PerCP-Cy5.5 and recognizes an epitope on the extracellular domain of CD45R across human, mouse, and cat species. CD45 is a member of the protein tyrosine phosphatase family involved in cell activation, growth, differentiation, and oncogenic transformation, with alternative splicing of three exons giving rise to up to eight isoforms of molecular masses in the range of 200–220 kDa. CD45R is expressed by the B cell lineage from early pro-B to mature B cells, with expression downregulated during differentiation to plasma cells, and is also present on subsets of T, NK, dendritic, and lymphokine-activated killer cells.

The antibody is supplied in a phosphate-buffered solution (pH 7.2) containing 0.09% sodium azide and 0.1% gelatin, and was purified by affinity chromatography. For flow cytometry, the suggested use is ≤ 0.25 µg per 1 x 10^6 cells in 100 µL. The product is stable at 2–8°C when stored undiluted; do not freeze and protect from prolonged exposure to light. This antibody clone has been verified for purity assessments of cells isolated with EasySep kits.