Anti-Ms CD43, R2/60, BT, 100µg

The R2/60 clone monoclonal antibody recognizes the mouse CD43 (leukosialin) surface antigen, a heavily glycosylated transmembrane sialoglycoprotein expressed on thymocytes, mature T lymphocytes, granulocytes, monocytes, macrophages, and the B-1a subset of B cells, while being absent from resting conventional B-2 B cells. This biotin conjugate from STEMCELL Technologies is supplied as a 100 µg vial of purified rat IgG2a, kappa immunoglobulin intended for research use only, supporting flow cytometry, immunofluorescence staining, and streptavidin-based cell separation workflows.

Direct immunofluorescent staining of splenocyte, thymocyte, or bone marrow suspensions typically uses approximately 1 µg of reagent per million cells, and the biotinylated format pairs cleanly with streptavidin-fluorophore secondaries in multicolor panels targeting T cell development, myeloid lineage commitment, and B-1 versus B-2 discrimination in murine autoimmunity, infection, and hematopoiesis models. The vial should be stored refrigerated and protected from light to preserve biotin activity across repeated experimental use.