Anti-Ms CD4, RM4-5, AZ, 25µg

This rat monoclonal IgG2a, kappa antibody, manufactured by STEMCELL Technologies and distributed by AFMS, targets mouse CD4 (also known as L3T4 or T4) and is conjugated to APC. The clone RM4-5 was raised in a Rat (DA) host against BALB/c mouse thymocytes and reacts with an ~55 kDa single-chain type 1 transmembrane glycoprotein belonging to the immunoglobulin superfamily, containing four extracellular Ig-like domains.

CD4 is expressed at relatively high levels by most thymocytes and on a subpopulation of T cells (T-helper/inducer cells), and at lower levels on dendritic cells; in the mouse it is not expressed by monocytes/macrophages. CD4 binds to a non-polymorphic region of MHC class II and acts as a co-receptor to the T cell receptor (TCR) in MHC II-restricted antigen recognition by enhancing the avidity of the association between the TCR and the MHC II-antigen complex. CD4 also amplifies signals from the TCR to the cytoplasm through interaction of its intracellular domain with cytoplasmic tyrosine kinases such as Lck. Binding of the RM4-5 antibody to CD4 inhibits ligand binding in vitro, and this binding can be blocked by the clone GK1.5 antibody.

Flow cytometry is the verified application, with a suggested use of ≤ 0.25 μg per 1 x 10^6 cells in 100 μL volume. The antibody has been used for purity assessments of cells isolated with EasySep™ kits, including the EasySep™ Mouse CD4+ T Cell Isolation Kit and the EasySep™ Mouse CD25 Regulatory T Cell Positive Selection Kit. It is purified by affinity chromatography and supplied in a phosphate-buffered solution (pH 7.2) containing 0.09% sodium azide. For storage, the product is stable at 2 - 8°C when stored undiluted, should not be frozen, and should be protected from prolonged exposure to light. Gene ID: 12504; for research use only.