Anti-Ms CD3e, 145-2C11, AZ, 100µg

This monoclonal antibody from STEMCELL Technologies, clone 145-2C11, targets the CD3ε (CD3e) subunit of the mouse T cell receptor (TCR)/CD3 complex. It is a hamster IgG1, kappa antibody designed for detection and manipulation of T cells. The antibody reacts with the ~20 kDa CD3ε subunit expressed on the surface of circulating mature T cells and NK-T cells, and variably on thymocytes. A majority of T cell neoplasms also express CD3.

The CD3 complex is assembled from combinations of CD3γ, δ, ε, η and ζ subunits, associates non-covalently with the TCR, and is involved in transducing antigen recognition signals into T cells and regulating cell surface expression of the TCR. Mutations in CD3 subunits have been associated with various immunodeficiency disorders including severe combined immunodeficiency (SCID).

The 145-2C11 antibody has been used for in vitro functional (blocking and activation) assays and has been reported to block binding by the clone 17A2 antibody. It is verified for cell isolation, flow cytometry, immunohistochemistry, and immunofluorescence, and is reported for use in ELISA, immunocytochemistry, immunoprecipitation, and Western blot. The antibody is not recommended for formalin-fixed, paraffin-embedded sections and has been verified for purity assessments of cells isolated with EasySep kits.