Anti-Ms CD267 (TACI), 8F10, AZ, 50µg

STEMCELL Technologies supplies a rat monoclonal IgG2a, kappa antibody (Clone 8F10) raised against mouse CD267, also known as TACI (TNFRSF 13B; transmembrane activator and CAML interactor). The reagent is conjugated to allophycocyanin (APC). CD267 is an approximately 30 kDa type III transmembrane protein of the TNF receptor superfamily (Gene ID: 57916). The antibody was generated using the extracellular domain of mouse CD267 recombinant protein as the immunogen.

CD267 is expressed primarily on the surface of maturing subsets of splenic B cells, including transition type 2 (T2) and marginal zone (MZ) B cells, as well as on plasma cells and plasmablasts, and its expression is upregulated on B cells exposed to LPS both in vitro and in vivo. The receptor plays important roles in humoral immunity by regulating B cell survival, proliferation, and maturation through interaction with the ligands APRIL and BAFF, stimulating activation of transcription factors including NF-κB. Ligand binding has been shown to induce either proliferation or apoptosis, consistent with a dual regulatory role, and CD267 also contributes to immunoglobulin isotype switching and B cell differentiation in response to thymus-independent antigens.

The antibody is verified for flow cytometry and has been validated for purity assessments of cells isolated with EasySep™ Mouse Pan-B Cell Isolation Kit and EasySep™ Mouse B Cell Isolation Kit. It was purified by column chromatography and formulated in phosphate-buffered saline (pH 7.2) containing 0.09% sodium azide and 0.1% gelatin. For flow cytometry, the suggested use is ≤ 0.25 µg per 1 × 10⁶ cells in 100 µL. The product is stable at 2–8 °C when stored undiluted; it should not be frozen and should be protected from prolonged exposure to light.