Anti-Ms CD25, 3C7, FI, 50µg

A FITC-conjugated rat monoclonal antibody from STEMCELL Technologies, Clone 3C7 (IgG2b, kappa), targets murine CD25 (interleukin-2 receptor α chain; IL-2Rα), an ~55 kDa type 1 transmembrane glycoprotein expressed on T and B cell progenitors and activated mature B and T cells. CD25 has been used as a marker to identify CD4+/FoxP3+ regulatory T cells in mice, and mutations in CD25 result in severe immunodeficiency, underscoring roles in lymphocyte differentiation, activation and proliferation, and in the maintenance of self-tolerance. The 3C7 antibody inhibits binding of IL-2 to free CD25 and the IL-2R receptor, and recognises an epitope distinct from those bound by the anti-CD25 clones 7D4 and PC61. CD25 itself has low affinity for its IL-2 ligand but forms dimers with CD122 (IL-2Rβ) and CD132 (IL-2Rγ) that together assemble the high-affinity IL-2R receptor.

The antibody has been verified for flow cytometry (FC) and is reported for FC, and Clone 3C7 is used for FC and cell separation. For flow cytometry the suggested use is ≤ 1 µg per 1 x 10^6 cells in 100 µL volume, and titration is recommended for optimal performance. The clone has been verified for purity assessments of cells isolated with EasySep™ kits, including the EasySep™ Mouse T Cell Enrichment Kit. The product is formulated in a phosphate-buffered solution at pH 7.2 containing 0.09% sodium azide, purified by affinity chromatography and conjugated with FITC under optimal conditions, and the solution is free of unconjugated FITC. The antibody is stable at 2–8°C when stored undiluted; it should not be frozen and should be protected from prolonged exposure to light.

Host species: Rat (LEW). Clonality: monoclonal. Isotype: IgG2b, kappa. Immunogen: IL-2-dependent BALB/c mouse helper T cell clone HT-2. Gene ID: 16184. Alternative names: IL-2Ralpha, Ly-43, p55, Tac. The product is for research use only and is not intended for human or animal diagnostic or therapeutic uses unless otherwise stated.