Anti-Ms CD24, M1/69, PE, 50µg

STEMCELL Technologies offers this PE-conjugated rat monoclonal antibody, clone M1/69, of the IgG2b, kappa isotype. It specifically recognizes an ~35 - 45 kDa glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed on lymphocytes, granulocytes, thymocytes, erythrocytes, epithelial cells, neurons, and dendritic cells. The immunogen used to generate the clone was C57BL/10 mouse splenic T cells and concanavalin A-activated splenocytes.

The target, CD24, is differentially expressed during T and B cell development and is not found on the majority of mature peripheral T cells or plasma cells. The reagent is verified and reported for flow cytometry (FC) applications and has been used for purity assessments of cells isolated with EasySep™ kits, including the EasySep™ Mouse B Cell Isolation Kit. For flow cytometry, the suggested use is ≤ 0.25 μg per 1 x 10^6 cells in 100 μL volume, with titration recommended for optimal performance for each application. The product is supplied in a phosphate-buffered solution at pH 7.2 containing 0.09% sodium azide and has been purified by affinity chromatography prior to PE conjugation, with the solution free of unconjugated PE.

For storage, the antibody is stable at 2 - 8°C when kept undiluted; it should not be frozen and should be protected from prolonged exposure to light. The M1/69 clone reportedly induces agglutination and lysis of mouse red blood cells in the presence of complement, and CD24 has been described as acting as an adhesion or co-stimulatory molecule involved in lymphocyte activation, proliferation and differentiation through homophilic interactions or by binding to ligands such as P-selectin (CD62P).