Anti-Ms CD24, M1/69, PE, 200µg

This PE-conjugated antibody from STEMCELL Technologies is a rat monoclonal IgG2b, kappa reagent directed against murine CD24, an approximately 35 - 45 kDa glycosylphosphatidylinositol (GPI)-anchored glycoprotein. The M1/69 clone reacts with CD24 expressed on lymphocytes, granulocytes, thymocytes, erythrocytes, epithelial cells, neurons and dendritic cells. CD24 is differentially expressed during T and B cell development, is found on pro-B, pre-B and mature B cells, and is strongly down-regulated after B cell activation. The molecule is involved in regulating B-cell apoptosis, in preventing terminal differentiation of B cells into antibody-secreting plasmablasts and plasma cells, and acts as an adhesion or co-stimulatory molecule through homophilic interactions or by binding to ligands such as P-selectin (CD62P).

The antibody was raised in rats (DA strain) using C57BL/10 mouse splenic T cells and concanavalin A-activated splenocytes as the immunogen, then purified by affinity chromatography and conjugated with PE; the solution is free of unconjugated PE. It is supplied in a phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Flow cytometry is a verified and reported application, and the clone has been used for purity assessments of cells isolated with EasySep™ kits, including the EasySep™ Mouse B Cell Isolation Kit. For flow cytometry the suggested use is ≤ 0.25 μg per 1 x 10⁶ cells in 100 μL volume, and titration is recommended for optimal performance in each application. The product is stable at 2 - 8 °C when stored undiluted; it should not be frozen and should be protected from prolonged exposure to light. The M1/69 antibody reportedly induces agglutination and lysis of mouse red blood cells in the presence of complement.