Anti-Ms CD19, 6D5, BT, 50µg

A biotin-conjugated rat monoclonal antibody from STEMCELL Technologies, Clone 6D5, targets CD19, an approximately 95 kDa type 1 transmembrane glycoprotein expressed on the surface of B cells throughout all stages of development, from early pre-B cells to plasma cells. Expression is down-regulated but persists in terminally differentiated plasma cells, and CD19 is also found on follicular dendritic cells.

By associating with CD21 and CD81, CD19 functions as a co-receptor for the B cell receptor and is involved in B cell activation and differentiation. Activation of CD19 is accompanied by phosphorylation of the cytoplasmic domain, which promotes binding to kinases and the induction of intracellular signaling cascades. Mutations in CD19 can result in severe immunodeficiency syndromes. Clone 6D5 recognizes the same epitope as clone 1D3 in cross-competition assays.

The antibody is of the IgG2a, kappa isotype and is also offered in additional conjugations, including Alexa Fluor 488, APC, FITC, PE, and unconjugated formats. It is suitable for cell isolation, flow cytometry, CyTOF, immunocytochemistry, immunofluorescence, immunohistochemistry, immunoprecipitation, and Western blot applications, and has been verified for purity assessments of cells isolated with STEMCELL Technologies EasySep kits, including the EasySep Mouse B Cell Isolation Kit.