Anti-Ms CD19, 1D3, UC, 100µg

From STEMCELL Technologies, the Anti-Mouse CD19 Antibody, Clone 1D3 is a rat (LEW) monoclonal IgG2a, kappa antibody specific for mouse CD19 and provided unconjugated. The 1D3 antibody recognizes an ~95 kDa type 1 transmembrane glycoprotein expressed on the surface of B cells throughout all stages of development, from early pre-B cells to plasma cells, with expression down-regulated but persisting in terminally differentiated plasma cells. CD19 is also found on follicular dendritic cells. By associating with CD21 and CD81, CD19 functions as a co-receptor for the B cell receptor and is involved in B cell activation and differentiation. Activation of CD19 is accompanied by phosphorylation of the cytoplasmic domain, promoting binding to kinases and the induction of intracellular signaling cascades. Mutations in CD19 can result in severe immunodeficiency syndromes. Clone 1D3 recognizes the same epitope as clone 6D5 in cross-competition assays. The antibody was raised against a recombinant mouse CD19-transfected cell line, with CD19 also known as B4 (Gene ID: 12478).

The antibody is supplied at 0.5 mg/mL in phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide, and was purified by column chromatography. The product is stable at 2 – 8 °C when stored undiluted; do not freeze. For flow cytometry, the suggested use is ≤ 0.5 μg per 1 x 10^6 cells in 100 μL, and the antibody should be titrated for optimal performance in each application. Flow cytometry is a verified application; functional assay, immunocytochemistry, immunofluorescence microscopy, immunohistochemistry, and immunoprecipitation are reported applications. This clone has also been verified for purity assessments of cells isolated with EasySep™ kits, including the EasySep™ Mouse B Cell Isolation Kit.