Anti-Ms CD138, 281-2, UC, 500µg

A rat monoclonal antibody from STEMCELL Technologies, Clone 281-2, reacts with an extracellular epitope on the core protein of mouse CD138 (Syndecan-1), a ~60 - 100 kDa type 1 transmembrane glycoprotein. CD138 is expressed on the surface of pre-B cells, immature B cells, and normal and malignant plasma cells (but not mature circulating B cells), as well as on non-hematopoietic cells including embryonic mesenchymal, endothelial, epithelial and neural cells. The target, also known by the alternative names B-B4, SDC1 and syndecan-1 (Gene ID: 20969), is thought to act primarily as a receptor that modulates cell proliferation, cell migration and cell-matrix associations by linking the extracellular matrix to the cytoskeleton; its heparin sulfate and chondroitin sulfate moieties associate with collagens, fibronectin, tenascin, thrombospondin and certain cytokines.

The antibody is of the IgG2a, kappa isotype and was raised against the Mouse NMuMG mammary gland epithelial cell line. It has been verified for flow cytometry (FC) and cell separation (CellSep), and is reported for functional assay (FA), immunocytochemistry (ICC), immunofluorescence microscopy (IF), immunohistochemistry (IHC), immunoprecipitation (IP) and Western blotting (WB). The clone has also been verified for purity assessments of cells isolated with EasySep kits, including the EasySep Mouse Pan-B Cell Isolation Kit and the EasySep Mouse CD19 Positive Selection Kit II. For flow cytometry, the suggested use is ≤ 1 μg per 1 x 10^6 cells in 100 μL volume, with titration recommended for optimal performance in each application.

The product is supplied unconjugated in a phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide, and was purified by affinity chromatography. It is stable at 2 - 8°C when stored undiluted; do not freeze.