Anti-Ms CD138, 281-2, PE, 25µg

This PE-conjugated antibody from STEMCELL Technologies is a rat monoclonal IgG2a, kappa reagent (clone 281-2) directed against mouse CD138 (Syndecan-1). The clone reacts with an extracellular epitope on the core protein of mouse CD138, a ~60 - 100 kDa type 1 transmembrane glycoprotein expressed on the surface of pre-B cells, immature B cells, and normal and malignant plasma cells, but not on mature circulating B cells. CD138 is also expressed on non-hematopoietic cells such as embryonic mesenchymal cells, endothelial, epithelial, and neural cells, where it is thought to act primarily as a receptor that modulates cell proliferation, cell migration, and cell-matrix associations by linking the extracellular matrix to the cytoskeleton. Heparan sulfate and chondroitin sulfate moieties attached to CD138 associate with several proteins, including collagens, fibronectin, tenascin, thrombospondin, and certain cytokines.

The antibody was raised against the mouse NMuMG mammary gland epithelial cell line and purified by affinity chromatography before conjugation with PE; the resulting solution is free of unconjugated PE and unconjugated antibody. It is supplied as a phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Flow cytometry (FC) is a verified application and cell separation (CellSep) is reported. The manufacturer suggests ≤ 0.25 μg per 1 x 10^6 cells in 100 μL volume for flow cytometry and recommends that the antibody be titrated for optimal performance in each application.

The reagent is intended for research use only and is not intended for human or animal diagnostic or therapeutic uses unless otherwise stated. It should be stored undiluted at 2 - 8°C; the product should not be frozen and should be protected from prolonged exposure to light. STEMCELL Technologies Inc.'s quality management system is certified to ISO 13485.