Anti-Ms CD138, 281-2, PE, 200µg

STEMCELL Technologies offers this rat monoclonal IgG2a, kappa antibody raised against mouse CD138 (Syndecan-1), clone 281-2, conjugated to PE. The antibody reacts with an extracellular epitope on the core protein of mouse CD138 (Syndecan-1), a ~60-100 kDa type 1 transmembrane glycoprotein expressed on the surface of pre-B cells, immature B cells, and normal and malignant plasma cells (but not mature circulating B cells). CD138 is also expressed on non-hematopoietic cells such as embryonic mesenchymal cells, endothelial, epithelial and neural cells.

CD138 is thought to act primarily as a receptor that modulates cell proliferation, cell migration and cell-matrix associations by linking the extracellular matrix to the cytoskeleton. Heparin sulfate and chondroitin sulfate moieties attached to CD138 associate with several proteins, including collagens, fibronectin, tenascin, thrombospondin and certain cytokines. The immunogen used was the mouse NMuMG mammary gland epithelial cell line. The antibody has been verified for flow cytometry and reported for use in cell separation, and has been verified for purity assessments of cells isolated with EasySep kits.

The product is formulated in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide, and was purified by affinity chromatography prior to PE conjugation. For flow cytometry, the suggested use is ≤ 0.25 μg per 1 x 10^6 cells in 100 μL volume. It is recommended that the antibody be titrated for optimal performance for each application. The product is stable at 2-8°C when stored undiluted; do not freeze, and protect from prolonged exposure to light. Products are for research use only and not intended for human or animal diagnostic or therapeutic uses unless otherwise stated.