Anti-Ms CD11b, M1/70, UC, 100µg

STEMCELL Technologies offers a rat monoclonal IgG2b, kappa antibody (clone M1/70) that recognizes CD11b, an ~170 kDa type I transmembrane glycoprotein also known as alphaM integrin, C3biR, CR3, Ly-40, Mac-1, and Mo1. The reagent is supplied unconjugated and is purified by affinity chromatography in phosphate-buffered saline (pH 7.2) containing 0.09% sodium azide. The antibody was raised against C57BL/10 mouse splenocytes and reacts with CD11b from human, mouse, rhesus, cynomolgus, baboon, chimpanzee, and rabbit.

CD11b associates non-covalently with CD18 to form the heterodimeric Mac-1 receptor, which engages ligands including ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, and fibrinogen. Through these interactions, Mac-1 mediates adherence of neutrophils and monocytes to stimulated endothelium and the phagocytosis of complement-coated particles. CD11b is expressed on granulocytes, monocytes, NK cells, dendritic cells, tissue macrophages, and subsets of T and B cells, and serves as a marker to distinguish naïve and memory CD8+ T cells. The M1/70 clone reportedly blocks iC3b binding to Mac-1.

Flow cytometry is a verified application, with a suggested use of ≤ 0.25 μg per 1 x 10^6 cells in 100 μL. Additional reported applications include CyTOF®, functional assay, FACS, flow cytometry, immunocytochemistry, immunofluorescence, immunohistochemistry, immunoprecipitation, and radioimmunoassay. The product is stable at 2 - 8°C when stored undiluted; it should not be frozen and remains stable until the expiry date on the label. According to the manufacturer's safety data sheet, the preparation is not classified as a hazardous substance or mixture under the Globally Harmonized System (GHS) or the 2012 OSHA Hazard Communication Standard, and no special personal protective equipment is required under normal use conditions.