Anti-Ms CD11b, M1/70, FI, 25µg

A monoclonal reagent from STEMCELL Technologies, this FITC-conjugated antibody (Clone M1/70, rat IgG2b, kappa) targets CD11b, an ~170 kDa type 1 transmembrane glycoprotein that associates non-covalently with CD18 to form the heterodimeric Mac-1 receptor. The antibody reacts with CD11b from human, mouse, rhesus, cynomolgus, baboon, chimpanzee, and rabbit. Through its interactions with ligands such as ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, and fibrinogen, Mac-1 functions in processes including the adherence of neutrophils and monocytes to stimulated endothelium and the phagocytosis of complement-coated particles.

CD11b is expressed on granulocytes, monocytes, NK cells, dendritic cells, tissue macrophages, and subsets of T and B cells, and has been used as a marker to distinguish naïve and memory CD8+ T cells. It is a relatively late marker for myeloid differentiation and is undetectable on most myelomonocytic hematopoietic progenitor cells and more primitive cells. The M1/70 clone reportedly blocks iC3b binding to Mac-1. The antibody was raised against C57BL/10 mouse splenocytes and purified by affinity chromatography before FITC conjugation; the formulation is phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide and 0.1% gelatin, and the solution is free of unconjugated FITC.

Verified applications include flow cytometry (FC); reported uses span FACS, FC, IF, and IHC. The reagent has been verified for purity assessments of cells isolated with EasySep™ kits, including the EasySep™ Mouse Monocyte Isolation Kit. For flow cytometry, the suggested use is ≤ 0.5 μg per 1 x 10^6 cells in 100 μL, and titration is recommended for optimal performance. The product is stable when stored undiluted at 2 - 8 °C; it should not be frozen and should be protected from prolonged exposure to light, remaining stable until the expiry date on the label.