Anti-Ms CD11b, M1/70, AZ, 25µg

A rat monoclonal IgG2b, kappa antibody from STEMCELL Technologies, clone M1/70, conjugated to APC and directed against CD11b. CD11b is an ~170 kDa type 1 transmembrane glycoprotein that associates non-covalently with CD18 to form the heterodimeric Mac-1 receptor. Through its interactions with ligands such as ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, and fibrinogen, Mac-1 functions in several processes, including the adherence of neutrophils and monocytes to stimulated endothelium and phagocytosis of complement-coated particles.

CD11b is expressed on the surface of granulocytes, monocytes, NK cells, dendritic cells, tissue macrophages, and subsets of T and B cells, and has been used as a marker to distinguish naïve and memory CD8+ T cells. It is a relatively late marker for myeloid differentiation and is undetectable on most myelomonocytic hematopoietic progenitor cells and more primitive cells. The M1/70 antibody reportedly blocks iC3b binding to Mac-1. The clone has been verified for purity assessments of cells isolated with EasySep™ kits, including the EasySep™ Mouse Monocyte Isolation Kit.

Species reactivity includes human, mouse, rhesus, cynomolgus, baboon, chimpanzee, and rabbit. The antibody was generated using C57BL/10 mouse splenocytes as the immunogen and purified by affinity chromatography. It is supplied in a phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide, free of unconjugated APC and unconjugated antibody. The product is stable at 2 – 8°C when stored undiluted; do not freeze and protect from prolonged exposure to light. For flow cytometry, the suggested use is ≤ 0.25 μg per 1 x 10^6 cells in 100 μL. The product appears as a clear liquid and is intended for research use only.