Anti-Ms CD11b, M1/70, AZ, 100µg

This rat monoclonal IgG2b, kappa antibody, manufactured by STEMCELL Technologies, recognizes CD11b (also referred to as αM integrin, C3biR, CR3, Ly-40, Mac-1, or Mo1), an approximately 170 kDa type 1 transmembrane glycoprotein that associates non-covalently with CD18 to form the heterodimeric Mac-1 receptor. Clone M1/70 was raised against C57BL/10 mouse splenocytes and is conjugated to APC (allophycocyanin).

The reagent reacts with human, mouse, rhesus, cynomolgus, baboon, chimpanzee, and rabbit samples. It is verified by the manufacturer for flow cytometry and has been used for purity assessments of cells isolated with EasySep™ kits, including the EasySep™ Mouse Monocyte Isolation Kit. CD11b is expressed on granulocytes, monocytes, NK cells, dendritic cells, tissue macrophages, and subsets of T and B cells, and has been used as a marker to distinguish naïve and memory CD8+ T cells. The antibody reportedly blocks iC3b binding to Mac-1.

The antibody is supplied in phosphate-buffered solution (pH 7.2) containing 0.09% sodium azide and is free of unconjugated APC and unconjugated antibody following affinity chromatography purification. For flow cytometry, the manufacturer suggests ≤ 0.25 μg per 1 × 10^6 cells in 100 μL, with titration recommended for optimal performance. Store at 2–8°C undiluted; do not freeze and protect from prolonged exposure to light. For research use only.